the regulatory maximum residue limit for ciprofloxacin in milk. Furthermore, the sensor offers rapid analysis
capability and potential portability, making it suitable for future on-farm and real-time dairy monitoring
applications without dependence on large laboratory infrastructure. Fig. 1 shows a dairy sensor for detection
of ciprofloxacin in milk samples. Overall, the study demonstrates that Lock-in Amplifier-based fluorescence
sensing technology provides a cost-effective, precise, and portable approach for antibiotic residue detection in
dairy products. The proposed method represents an important advancement toward automated and intelligent
dairy quality inspection systems, supporting food safety regulations and public health protection.
2.3 Development of spectroscopic sensor system for an IOT application of adulteration identification on
milk using machine learning
Sowmya and Ponnusamy focuses on the design and development of an IoT-enabled multispectral sensor system
integrated with machine learning algorithms for rapid and real-time detection of milk adulteration.
[16]
The
research addresses the limitations of traditional chemical testing methods, which are often time-consuming,
expensive, laboratory-dependent, and unsuitable for field-level applications. The developed system integrates
affordable multispectral sensors covering wavelengths from 410 nm to 940 nm with an Arduino-based
microcontroller to capture optical spectral responses from milk samples. Various adulterants such as sodium
salicylate, dextrose, hydrogen peroxide, and ammonium sulphate were tested to generate a spectral fingerprint
dataset. The collected spectral data were analyzed using machine learning algorithms including Naive Bayes,
Support Vector Machine (SVM), Decision Tree, Linear Discriminant Analysis (LDA), and Neural Network models
for adulteration classification. The system provides a non-destructive and cost-effective solution for milk quality
monitoring through an IoT-enabled cloud platform, allowing remote monitoring and real-time visualization of
results through a web interface. This integration improves transparency, portability, and digital traceability for
dairy producers and regulatory authorities without requiring advanced laboratory infrastructure. The
developed prototype is portable and economically feasible, with an approximate system cost of INR 8,624,
making it suitable for rural and small-scale dairy environments. Overall, the study demonstrates that IoT-
integrated spectroscopic sensing combined with artificial intelligence can provide an intelligent, real-time, and
reliable solution for milk adulteration detection and food safety management.
2.4 IoT integrated fuzzy classification analysis for detecting adulterants in cow milk
Lal et al. presents an IoT-enabled multispectral sensor system for rapid milk adulteration detection.
[17]
The
system uses AS7265X spectral sensors and an Arduino microcontroller to collect milk spectral data across
wavelengths from 410 nm to 940 nm. Four adulterantssodium salicylate, dextrose, hydrogen peroxide, and
ammonium sulphatewere analyzed using machine learning techniques. Algorithms such as Naive Bayes, SVM,
Decision Tree, LDA, and Neural Networks were applied for classification. The neural network achieved up to
100% accuracy after hyperparameter tuning using a genetic algorithm. The proposed system is non-destructive,
portable, and cost-effective, with a total cost of about INR 8,624. IoT integration enables real-time monitoring
and remote visualization of adulteration results through a web interface. The study demonstrates that
multispectral sensing and artificial intelligence can provide an efficient solution for dairy quality monitoring
and food safety management.
2.5 Milk Chain: A blockchain-enabled dairy traceability platform
Nukapeyi et al. present a blockchain-
challenges such as milk adulteration, poor traceability, and quality degradation in global dairy supply chains.
[18]
The study highlights the limitations of traditional manual monitoring methods, which can lead to health risks
and economic losses, especially in densely populated regions such as India. The proposed system integrates IoT
sensors, blockchain technology, and machine learning to ensure transparency, tamper-proof data management,
and reliable quality monitoring. The platform focuses on preserving nutritional quality, detecting adulteration,
supporting dairy farmers economically, and ensuring product authenticity. Various milk quality parameters,
including pH, temperature, taste, odor, fat content, turbidity, color, and grade, were analyzed using the CatBoost
machine learning algorithm. The findings identify critical factors affecting milk quality and demonstrate the
potential of intelligent digital technologies in building a secure and sustainable dairy supply chain system.
2.6 Portable multispectral milk quality monitoring using machine learning
Sharma et al. developed a non-destructive, AI-assisted spectro-analytical system for rapid milk adulteration
detection using a portable reflectance spectrophotometer operating in the 410940 nm wavelength range.
[19]
The study evaluated six adulterants-urea, formalin, hydrogen peroxide, baking soda, sucrose, and cornstarch
to establish compositional and spectral relationships. Physicochemical analysis showed significant variations in
SNF, fat, and protein content due to adulteration. Spectral analysis identified important wavelength regions
associated with chromophore absorption and near-infrared (NIR) responses. Principal Component Analysis
(PCA) confirmed clear adulterant-specific clustering, while ANOVA-based feature selection identified key